Simultaneous inhibition of ID1 and ID3 mitigates fibroblast activation via cell cycle and MEK/ERK pathways in pulmonary fibrosis
Background: . Methods: to determine the role of ID1/ID3 in pulmonary fibrosis. Results: . Lung-specific inhibition of ID1/ID3 using adeno-associated viruses expressing short hairpins targeting ID1 and ID3 also reversed pulmonary fibrosis in mice. Mechanistically, ID1/ID3 inhibition reduced fibroblast proliferation through regulation of cell cycle genes and attenuated fibroblast differentiation via the MEK/ERK pathway. Conclusions: Simultaneous inhibition of ID1 and ID3 attenuates pulmonary fibrosis. Targeting ID1/ID3 represents a potential novel therapeutic strategy for IPF.
Authors
- Diego Fraidenraich (ORCID: https://orcid.org/0000-0001-5369-4446)
- Giovanni Ligresti (ORCID: https://orcid.org/0000-0002-3375-8665)
- Eric Mensah
- Michael Aziz (ORCID: https://orcid.org/0000-0001-9873-9759)
- Mark C. Renton (ORCID: https://orcid.org/0000-0003-0909-7948)
- Yassine Sassi (ORCID: https://orcid.org/0000-0002-7807-1728)
- Aanandi Parashar
- Robert Benezra (ORCID: https://orcid.org/0000-0002-6031-6870)
- Ahmed Raslan (ORCID: https://orcid.org/0000-0003-4936-1459)
- Aymen Halouani
- Jacob Dahlka
- Seun Imani
- Samar A Antar
- Scott R. Johnstone
Institutions
- Rutgers, The State University of New Jersey (US)
- Boston University (US)
- Virginia Western Community College (US)
- Damietta University (EG)
- Memorial Sloan Kettering Cancer Center (US)
- Virginia–Maryland College of Veterinary Medicine (US)
- Roanoke College (US)
- Carilion Clinic (US)
- Virginia Tech (US)
- Assiut University (EG)
- Icahn School of Medicine at Mount Sinai (US)
Publication Details
- Journal
- Theranostics
- Published
- 2026-04-16
- DOI
- https://doi.org/10.7150/thno.127118
- Primary Topic
- TGF-β signaling in diseases
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- American Heart Association
- National Institutes of Health