Фармакологічна дія густого екстракту квітів пижма звичайного (Tanacetum vulgare L.) на експериментальній моделі естроген-індукованого холестазу

The aim. To study the protective effect of thick extract of Tanacetum vulgare L. flowers (TETVF) in an experimental model of estrogen-induced cholestasis (EIC). Materials and methods. EIC was reproduced by subcutaneous administration of 7α-ethinylestradiol (E) (5 mg/kg) to rats. TETVF and the reference preparation (RP) cholelesan (ChL) were administered to animals intragastrically once a day for 3 days before and 5 days of the modeling period. One day after the last E administration, the animals were anesthetized (thiopental sodium, 50 mg/kg, intraperitoneally), the volume of bile secreted in 60 min was determined, and the bile secretion rate (BSR) was calculated. The activity of alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma-glutamyl transpeptidase (GTT), the content of TBA-active products (TBA-AP) and reduced glutathione (RG) was determined in the blood serum. For histological examination, prepared liver sections were stained with hematoxylin, eosin and sudan IV to identify lipids. A semi-quantitative assessment of EIC signs was performed. Results of the study. In animals with EIC TETVF contributed to the normalization of bile volume and BSR to the level of intact control (IC), a significant (p < 0.05) decrease in the studied parameters: ALT, LF and GTT, a decrease in lipid peroxidation (LPO) processes and an increase in liver antioxidant defense, as evidenced by a significant decrease in the level of TBA-AP (30.98±0.90 μmol/g vs. 44.87±4.26, p < 0.05) and a 1.9-fold increase in RG (4.11±0.36 μmol/g vs. 2.19±0.16, p < 0.05) compared to the control pathology (CP). In general, TETVF showed a normalizing effect like RP, but was inferior to it in terms of its effect on BER and on ALT activity and TBA-AP level. After the introduction of TETVF the severity of ductal proliferation significantly decreased by 1.6 times (p < 0.05), periductal inflammation by 3.8 times (p < 0.05), and fatty degeneration of hepatocytes of the periductal zones by 2.3 times (p < 0.05) compared to CP. Conclusion. The ability of TETVF to improve impaired liver function under conditions of EEIS and reduce pathological manifestations was established