Magnetic Graphene for High-Performance Isolation of Bone Marrow Mesenchymal Stem Cell-Derived Exosomes via Ca <sup>2+</sup> -Dependent Reversible Recognition
experiments confirmed that BMSC-Exos isolated via TIM4@MGO markedly promoted L929 cell proliferation and accelerated scratch wound healing. Proteomic profiling further revealed that BMSC-Exos isolated by TIM4@MGO were significantly enriched in proteins associated with extracellular matrix remodeling and the TNF signaling pathway, implying their potential role in fibrosis regulation. In a mouse pulmonary fibrosis model, compared with those isolated by ultracentrifugation and polyethylene glycol precipitation methods, TIM4@MGO-isolated BMSC-Exos exerted superior therapeutic efficacy in mitigating lung tissue structural damage and attenuating fibrotic progression. Collectively, this study establishes a high-efficiency, high-purity, and reusable exosome isolation technique, offering robust technical support for both fundamental research and the clinical translation of BMSC-Exo therapeutics.
Authors
- Jinxiao Yan
- 康园超
- Hongrui Ji
- He Li (ORCID: https://orcid.org/0000-0001-6429-9097)
- Shiyu Liu (ORCID: https://orcid.org/0000-0003-0208-8635)
- H Yang
- Shuoqi Tian
- Yanwen Hao
- Shizhe Chen
- Ju Huang
- Ning Cui
Institutions
- Northwestern Polytechnical University (CN)
- Center for Advanced Biomaterials for Healthcare (IT)
- Shanghai Second People's Hospital (CN)
- Biomechanics Institute of Valencia (ES)
- Northwest Institute For Non-Ferrous Metal Research (CN)
- Ministry of Education (TH)
- Kai Biotech (South Korea) (KR)
Publication Details
- Journal
- ACS Nano
- Published
- 2026-05-01
- DOI
- https://doi.org/10.1021/acsnano.6c02520
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Fundamental Research Funds for the Central Universities