Isolation And Screening of Soil Fungi on Different Culture Media

In the present investigation an attempt has been made to isolate the soil fungi from different localities. For isolation of fungi soil samples was collected from the different fields in pre sterilised zip lock bags. Soil samples were cultured on the different culture media like Potato dextrose agar, Rose Bengal agar, Czapek dox agar and Glucose nitrate agar by serial dilution method. The culture plates were incubated at 300c temperature. Fungi were sub cultured to obtain pure culture after getting pure cultures screening of fungi were done on different media for different parameters and fungi were identified by standard protocol. PDA supported the highest diversity and fastest growth, suggesting medium composition significantly influences fungal isolation and cultivation.