Screening of Phytochemicals and Estimation of Secondary Metabolites in Plant Species Euphorbia neriifolia L. and Caralluma fimbriata Wall.

The active components that give medicinal plants their therapeutic properties are phytochemicals, which are usually secondary metabolites like alkaloids, steroids, flavonoids, terpenoids, and tannins. Portions of the therapeutic herbs Caralluma fimbricata and Euphorbia neriifolia were investigated for secondary metabolites. Different techniques were used to estimate each secondary metabolite, such as the aluminium chloride colorimetric method for flavonoids, the Folin-Ciocalteu method for phenol, and the Folin-Denis method for tannin. Euphorbia neriifolia has the highest levels of flavonoids, tannin, and phenol. Caralluma fimbricata on the other hand, had a lower phenol content. It will be necessary to employ robust analytical separation and detection techniques like GCMS and HR-LCMS in future studies on secondary metabolites.