Sensitive and Robust One-Pot RPA-CRISPR/Cas12a Assay with Elimination of <i>cis</i> -Cleavage
and Avian Leukosis Virus subgroup J (ALV-J) samples. Overall, the 11bp+9ss one-pot assay exhibits superior sensitivity and robustness, showing great potential for POCT of bacteria and viruses.
Authors
- Xiaochuan Tang (ORCID: https://orcid.org/0000-0001-9325-3434)
- X Wang
- Yingying Zhao (ORCID: https://orcid.org/0000-0002-6170-6125)
- Ruiying Han (ORCID: https://orcid.org/0009-0008-2383-3087)
- Zhirui Wu
- Ning Xiao
Institutions
- Guangxi University (CN)
- Kerala Veterinary and Animal Sciences University (IN)
- Guangxi Academy of Sciences (CN)
Publication Details
- Journal
- Analytical Chemistry
- Published
- 2026-05-01
- DOI
- https://doi.org/10.1021/acs.analchem.5c06674
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Natural Science Foundation of Guangxi Zhuang Autonomous Region