Enzymatic Access to Norstatines by PLP-Dependent Decarboxylative C–C Bond Formation Involved in Leuhistin Biosynthesis
deprotonation. This suggests the catalytic mechanism is distinct among the canonical PLP-dependent decarboxylative C-C bond forming enzymes. Genome mining of LhnE homologues also implicated the existence of previously unknown norstatine natural products. Furthermore, LhnE was successfully applied to the biocatalytic synthesis of non-natural norstatines. Together, this study provides novel insights into PLP-dependent C-C bond formation and establishes a biocatalytic route for streamlined access to norstatine pharmacophores.
Authors
- Takayoshi Awakawa (ORCID: https://orcid.org/0000-0003-2575-3175)
- Y. Yamada
- Taku Mizutani (ORCID: https://orcid.org/0000-0002-6483-2901)
- Naruhiko Adachi
- Ikuro Abe
- Takahiro Mori
Institutions
- Center for Disease Dynamics, Economics & Policy (US)
- National Sun Yat-sen University (TW)
- University of Tsukuba (JP)
- Tohoku University (JP)
- National Synchrotron Radiation Research Center (TW)
- RIKEN Center for Sustainable Resource Science (JP)
- Institute of Natural Science (KP)
- University of Toyama (JP)
- The University of Tokyo (JP)
- Kumamoto University (JP)
Publication Details
- Journal
- Journal of the American Chemical Society
- Published
- 2026-05-01
- DOI
- https://doi.org/10.1021/jacs.6c05214
- Primary Topic
- Microbial Natural Products and Biosynthesis
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Institute for Fermentation, Osaka
- Japan Agency for Medical Research and Development
- Noda Institute for Scientific Research
- University of Tsukuba
- Shorai Foundation for Science and Technology
- Japan Society for the Promotion of Science