Identification of multiple arbovirus infections/exposure in Northeastern Brazil using a multiplex microsphere immunoassay
= 374) to evaluate the sensitivity/specificity of our multiplex IgG MIA. We reported high sensitivity of nonstructural protein 1 (NS1)-based IgG MIA (94.4%-100% except YFV NS1) and overall low specificity of ZIKV and WNV NS1 due to cross-reactivities from repeated orthoflavivirus infections, including secondary DENV and DENV + ZIKV infections. Using our algorithm and confirmatory tests, we reported multiple arbovirus infections or exposure (DENV + ZIKV, DENV + YFV, DENV + ZIKV + YFV, and/or CHIKV) in the majority of participants from a town in Northeastern Brazil.
Authors
- Eduardo Martins Netto (ORCID: https://orcid.org/0000-0003-1691-6761)
- Yu-Ching Dai (ORCID: https://orcid.org/0009-0008-4855-0374)
- Melissa Barreto Falcão (ORCID: https://orcid.org/0000-0003-0668-5525)
- Rennsilve C. Salomon
- Ricardo Khouri (ORCID: https://orcid.org/0000-0001-5664-4436)
- Jih‐Jin Tsai (ORCID: https://orcid.org/0000-0003-2226-8916)
- Szu‐Chia Hsieh
- Célia Pedroso
- Rafaela Mayoral
- Viviane Boaventura (ORCID: https://orcid.org/0000-0002-7241-6844)
- Laise Eduarda Paixão de Moraes (ORCID: https://orcid.org/0000-0001-9095-5860)
- Róger Costa
- Carlos Brites
- Guan-Hua Chen
- Wei-Kung Wang
Institutions
- University of Hawaiʻi at Mānoa (US)
- Universidade Federal da Bahia (BR)
- Kaohsiung Medical University (TW)
- Universidade Estadual de Feira de Santana (BR)
- Kaohsiung Medical University Chung-Ho Memorial Hospital (TW)
- Fundace (BR)
- Fundação Oswaldo Cruz (BR)
Publication Details
- Journal
- Journal of Clinical Microbiology
- Published
- 2026-04-30
- DOI
- https://doi.org/10.1128/jcm.00013-26
- Primary Topic
- Respiratory viral infections research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- National Institute of Allergy and Infectious Diseases