Nano‐Encapsulated Vitamin E Enhances Cryo‐Resistance of Stallion Spermatozoa: A Comprehensive Evaluation of Sperm Quality, Kinematic Parameters, Antioxidant Capacity, Microbiota and Ultrastructure
) in all treated semen samples (p < 0.01). VEN (1 mM) significantly reduced the proportion of apoptotic sperm (p < 0.01), increased viable sperm counts and the preservation of sperm ultrastructural integrity, as evidenced by intact acrosome and plasma membranes. VE and VEN treatments markedly lowered the total bacterial and coliform counts. Overall, supplementation with VEN (0.1 and 1 mM) effectively optimizes post-thaw stallion sperm quality by enhancing antioxidant defences and reducing the seminal microbiota.
Authors
- Mohamed K. Derbala (ORCID: https://orcid.org/0000-0002-5757-1483)
- Wael A. Khalil (ORCID: https://orcid.org/0000-0001-9539-3246)
- Hesham E. Mostafa
- Mahmoud A E Hassan
- Sameh A Abdelnour
- Mostafa A El-Harairy
Institutions
- Mansoura University (EG)
- Zagazig University (EG)
- Ministry of Agriculture (LV)
- Agricultural Genetic Engineering Research Institute (EG)
- Animal Health Research Institute (TW)
Publication Details
- Journal
- Reproduction in Domestic Animals
- Published
- 2026-04-30
- DOI
- https://doi.org/10.1111/rda.70213
- Primary Topic
- Sperm and Testicular Function
- Type
- article
- Field-Weighted Citation Impact
- 0.00