Anxiolytic and Memory Protective Effects of Withanolide D Isolated From <i>Acnistus arborescens</i> in Adult Zebrafish
receptor, supporting a putative allosteric interaction in a functionally related modulatory region rather than definitive occupation of the classical diazepam site. Consistently, normal mode analysis (NMA) showed that withanolide D increases receptor mobility compared to diazepam, with RMSF values reaching up to 0.98 Å, indicating enhanced structural flexibility and dynamic modulation of the protein. Thus, these findings suggest that withanolide D has therapeutic potential for the treatment of anxiety, in addition to providing protective effects on memory.
Authors
- Matheus Nunes da Rocha (ORCID: https://orcid.org/0000-0002-0929-4565)
- Jéssica Bezerra Maciel (ORCID: https://orcid.org/0000-0002-8626-1257)
- Pedro Henrique Jataí Batista (ORCID: https://orcid.org/0000-0002-7185-4981)
- Alexandre Magno Rodrigues Teixeira (ORCID: https://orcid.org/0000-0001-6786-2076)
- Otília Deusdênia Loiola Pessoa (ORCID: https://orcid.org/0000-0002-1617-7586)
- Maria Kueirislene Amâncio Ferreira (ORCID: https://orcid.org/0000-0002-4270-8109)
- Joilna Alves Da Silva
- Cléia Rocha de Sousa Feitosa
- Ivana Carneiro Romão (ORCID: https://orcid.org/0000-0002-6151-9397)
- Thaís Rocha Cavalcante (ORCID: https://orcid.org/0000-0002-2646-6453)
- Jane Eire Silva Alencar de Menezes (ORCID: https://orcid.org/0000-0001-9632-5464)
- Emmanuel Silva Marinho (ORCID: https://orcid.org/0000-0002-4774-8775)
- Andreia Ferreira de Castro Gomes (ORCID: https://orcid.org/0000-0002-0567-064X)
- Francisco Anderson Nascimento Barros
- Márcia Machado Marinho (ORCID: https://orcid.org/0000-0002-7640-2220)
- A G Da Silva
- Hélcio Silva Dos Santos
- Nicole de Abreu Bandeira
Institutions
- Universidade Estadual do Ceará (BR)
- Universidade Federal do Ceará (BR)
- State University of Vale do Acaraú (BR)
- Universidade Regional do Cariri (BR)
- University of Minho (PT)
Publication Details
- Journal
- Chemistry & Biodiversity
- Published
- 2026-04-30
- DOI
- https://doi.org/10.1002/cbdv.202502272
- Primary Topic
- Phytochemicals and Medicinal Plants
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico