Inactivation of NONO by Auranofin or RNA Interference Triggers Lethal Oxidative Stress in Neuroblastoma Cells

BACKGROUND: oncogene amplification and is particularly aggressive and therapy-resistant, underscoring the need for novel therapeutic targets. METHODS: Gene knockdown with siRNAs, qRT-PCR, flow cytometry-assisted measurements of intracellular oxidation and cell death parameters, immunoblotting, cytotoxicity assays (MTT, colony formation). RESULTS: -amplified cells demonstrated a significantly suppressed clonogenic survival 14 days after transient exposure to these combinations compared with each agent alone; HS5 fibroblasts were largely spared. CONCLUSIONS: -amplified tumors vulnerable to oxidative stress.