Potential role of anaerobic plant-associated bacteria in naphthenic acid degradation

rhizosphere. The strongest relationships between microbial taxa and the decrease in NAFC concentrations were for anaerobic bacteria, suggesting a prominent role for these bacteria in NAFC degradation in CWTS. This study highlights potential microbial targets for improving CWTS efficiency for NAFC detoxification during OSPW remediation. IMPORTANCE: Naphthenic acid fraction compounds (NAFCs) represent one of the most toxic and persistent contaminants in oil sands process-affected water (OSPW), posing a significant environmental challenge. As oil sands operations continue, large volumes of OSPW have accumulated, requiring effective treatment before any potential environmental discharge. Our study explores bioremediation through constructed wetland systems as a sustainable approach to reduce NAFC concentrations. By uncovering the microbial dynamics involved in NAFC degradation, our research provides critical insights into the identification of microbial communities that can enhance the remediation process. This manuscript contributes to advancing the scientific foundation needed to develop effective, biological solutions for OSPW treatment.