Study of lavender essential oil encapsulated chitosan-derivative nanoparticles on shampoo applications

Since lavender essential oil (LEO) contains antibacterial, antiinflammatory, and calming qualities, it is commonly utilized in hair care products. However, adding LEO conventionally results in quick evaporation and a shorter product shelf life due to its high volatility. In this study, chitosan-derivative nanoparticles (CSNPs) were initially prepared by degradation, modification, and purification. LEO was then encapsulated to create LEO-CSNPs, and FTIR, DLS, TEM, and 'H-NMR were used to evaluate both CSNPs and LEO-CSNPs. The effectiveness of the shampoos was next assessed by clinical trials and in vitro tests, such as malassezia inhibition, sebocyte lipid secretion, DPPH radical scavenging as well as qPCR for inflammatory markers with varying concentrations of LEO-CSNPs. The findings confirmed successful encapsulation since LEO-CSNPs (352.27 nm) had a higher particle size than CSNPs (218 nm). With a 15.5 mm Malassezia inhibition zone, 87.71% sebocyte lipid inhibition, 79.03% DPPH scavenging rate, and a notable downregulation of IL-6 and IL-8 expression. Meanwhile, the 0.5% LEO-CSNPs group demonstrated concentration-dependent efficacy. In terms of clinical results, fragrance longevity was increased to 7.2 hours, scalp flaking was decreased by 77.73%, and itching was also decreased by 69.05%. In conclusion, encapsulating LEO in chitosan-derived nanoparticles resolved its volatility problem and improved the antifungal, sebum-regulating, antioxidant, and anti-inflammatory properties in shampoo. Finally, the ideal concentration of LEO-CSNPs is between 0.3% and 0.5%, laying the groundwork for multipurpose shampoo.