In situ biomimetic near-infrared fluorescent protein formed by tumor signature proteins for detecting breast cancer biopsy

to tumor signature proteins, forming biomimetic near-infrared fluorescent proteins (NIR-FPs) with much-enhanced brightness. This approach effectively illuminates the tumor region for rapid and accurate identification. The tumor-selective dye inserts into the pockets of tumor signature proteins, then covalently binds with the -SH group of the protein, resulting in a series of NIR-FPs. By combining this with three-dimensional histological electrophoresis, we can select specific molecular weight layers of NIR-FPs (labeled tumor signature proteins) to accurately determine tumor margins. When integrating with the high-throughput 40 × 40 microwell array module, our system demonstrates excellent diagnostic accuracy in processing smaller puncture biopsy specimens. Moreover, the alkyne group in the dye facilitates proteomics analysis of all tumor signature proteins captured by the tumor-selective dye, providing valuable information for identifying new targets for tumor detection.