From Congestion to Clarity: On the Complementarity of Resolving Power and Spectral Simplification for Intact Protein Characterization
200) and further to 97.7% when PTCR was applied. This approach was applied to the characterization of biopharmaceuticals by analyzing the three digested and disulfide-reduced ∼25 kDa subunits of the NIST monoclonal antibody (mAb) on a liquid chromatography time scale.
Authors
- Jake Kline (ORCID: https://orcid.org/0000-0001-7264-7637)
- Graeme C. McAlister
- Kenneth R. Durbin (ORCID: https://orcid.org/0000-0001-7163-4877)
- Luca Fornelli (ORCID: https://orcid.org/0000-0001-6334-1046)
- Rafael D. Melani (ORCID: https://orcid.org/0000-0002-0349-235X)
- Linda Lieu
- Christopher Mullen
- David Bergen
- Jingjing Huang
Institutions
- Thermo Fisher Scientific (United States) (US)
- University of Oklahoma (US)
Publication Details
- Journal
- Journal of the American Society for Mass Spectrometry
- Published
- 2026-04-27
- DOI
- https://doi.org/10.1021/jasms.6c00016
- Primary Topic
- Protein purification and stability
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Institute of General Medical Sciences