An Ene Reductase, KlebER1, for the Production of Dihydrocarvone: Identification, Characterization, Engineering, and Application
for dihydrocarvone production. This biocatalytic system achieved a dihydrocarvone concentration of 5.88 mmol/L and a conversion rate of 99%, representing a significant improvement compared to the single use of A303N mutant. This study lays the foundation for investigating the function and modification of ERs and promoting the efficient biosynthesis of dihydrocarvone.
Authors
- L F Zhang
- Jie Yang (ORCID: https://orcid.org/0000-0003-4801-7162)
- Nannan Wang (ORCID: https://orcid.org/0000-0001-8261-2323)
- Wanying Wang (ORCID: https://orcid.org/0009-0001-9973-5466)
- Li Z
- Kaijing Yin
- Zhenzhen Huang
- Yongming Xu
- Yingjie Feng
Institutions
- China Tobacco (CN)
- Henan University of Technology (CN)
- Food Research Institute (SK)
- Technical and Vocational University (IR)
- Institute of Food Science & Technology (GB)
Publication Details
- Journal
- Journal of Agricultural and Food Chemistry
- Published
- 2026-04-27
- DOI
- https://doi.org/10.1021/acs.jafc.5c16339
- Primary Topic
- Enzyme Catalysis and Immobilization
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Henan University of Technology
- China National Tobacco Corporation
- Education Department of Henan Province