Humanin improved the rotenone-induced reactive oxygen species formation in PC12 cells by modulating the SIRT3/Nrf2/HO-1 signaling pathway
/NADH ratio. It also increased the expression of SIRT3, Nrf2, HO-1, and NQO1 proteins and decreased the expression of Ac-SOD protein under rotenone exposure. At the same time, it also activated the Nrf2/HO-1 signaling pathway, which depends on HN-mediated SIRT3 activation.ConclusionThese results suggest that HN plays a protective role in rotenone-induced neurotoxicity by suppressing oxidative stress and activating the antioxidant response via the Nrf2/HO-1 pathway, which is regulated by SIRT3 in PC12 cells.
Authors
- Yan Sai (ORCID: https://orcid.org/0000-0002-5520-0443)
- Yaohui Shan (ORCID: https://orcid.org/0000-0003-1516-2822)
- Qifu Zhang
- X Liu
- Wei Ge (ORCID: https://orcid.org/0009-0002-3251-6143)
Institutions
- Army Medical University (CN)
Publication Details
- Journal
- Toxicology and Industrial Health
- Published
- 2026-04-27
- DOI
- https://doi.org/10.1177/07482337261445494
- Primary Topic
- GDF15 and Related Biomarkers
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China