Directed Evolution of Glucosyltransferase ZmDo and Its Application in the Synthesis of Luteolin-5-O-Xyloside
), 2.3-fold higher than the wild type. A de novo UDP-Xyl biosynthetic pathway was constructed, enabling intracellular cofactor regeneration. Under optimized conditions, a titer of 4451 mg/L with an 87% conversion rate was achieved.
Authors
- Lei Yao (ORCID: https://orcid.org/0000-0001-9546-8789)
- Jiaqi Xu (ORCID: https://orcid.org/0000-0002-2774-5016)
- Changning Lu
- Wuyang Huang
- Linguo Zhao (ORCID: https://orcid.org/0009-0005-8102-7970)
- Jing Wang
- Qi Li
Institutions
- Nanjing Forestry University (CN)
- Jiangsu Academy of Agricultural Sciences (CN)
- Jingchu University of Technology (CN)
- Institute of Agro-Products Processing Science and Technology (CN)
- Forest Research (GB)
Publication Details
- Journal
- Journal of Agricultural and Food Chemistry
- Published
- 2026-04-27
- DOI
- https://doi.org/10.1021/acs.jafc.6c01223
- Primary Topic
- Carbohydrate Chemistry and Synthesis
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Key Research and Development Program of China