A microvolume protocol for catalase activity
. Our results demonstrate that the protocol accurately evaluates CAT activity, reflecting the physiological and metabolic characteristics of the analyzed tissues. To ensure specificity, CAT inhibitors and protein denaturation controls were employed. Overall, this methodology expands experimental possibilities for assessing enzymatic activity in reduced biological models and investigating their responses to oxidative stress.
Authors
- João Batista Teixeira da Rocha (ORCID: https://orcid.org/0000-0003-3829-0595)
- Élgion L. S. Loreto (ORCID: https://orcid.org/0000-0002-7586-8168)
- Beatriz Corrêa da Rocha (ORCID: https://orcid.org/0009-0000-6223-0280)
- Marcos Trindade da Rosa Rosa
Institutions
- Universidade Federal de Santa Maria (BR)
Publication Details
- Journal
- Toxicology Mechanisms and Methods
- Published
- 2026-04-27
- DOI
- https://doi.org/10.1080/15376516.2026.2645750
- Primary Topic
- Enzyme Catalysis and Immobilization
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Conselho Nacional de Desenvolvimento Científico e Tecnológico