Assessment of Amaranthus hypochondriacus L. efficacy on oxidative stress and reproductive parameters in fipronil intoxicated male albino rats

The therapeutic potential of Amaranthus hypochondriacus L. attributed to their diverse phytochemical makeup. Earlier studies have identified various bioactive phytoconstituents such as flavonoids, terpenoids, tannins, saponins, phytosterols and phenols in different parts of plant, including flower, seeds, leaves and stems. These phytochemicals have therapeutic importance, especially antioxidant, anti-inflammatory and cholesterol-lowering properties. The antioxidant activity of flavonoids and phenols could help the protection of oxidative damage. Although direct research on the therapeutic application of A. hypochondriacus treating reproductive disorders is limited, their active components suggest promising benefits. Further studies are required to determine their efficacy, appropriate dosage, safety parameters and underlying mechanisms of action. The aim of this study was to evaluate the A. hypochondriacus seed extract potential on oxidative and reproductive parameters in fipronil-intoxicated male Wistar rats. In the current investigation, group I given normal feed and water served as control, group II was administered with fipronil (at 24.25 mg/kg body weight), in hydroethanolic seed extract of A. hypochondriacus (HSEAH) (at 100 mg/kg body weight) in group III and combination of Fipronil (at 24.25 mg/kg body weight) and HSEAH (at 100 mg/kg body weight) was administered in group IV for 90 days in rats followed by estimating the parameters related to oxidative stress and reproductive toxicity parameters and histopathological examination. The results of study showed HSEAH treatment significantly (p<0.05) reduced the toxicosis caused by oxidative stress and elevated the levels of antioxidant enzymes such as reduced glutathione (GSH), lipid peroxidation (LPO) and catalase (CAT) in the various vital organ tissues. The present study offers an opportunity to explore the potential therapeutic properties of A. hypochondriacus in combating fipronil induced oxidative stress and reproductive toxicity.