Simultaneous Electrochemical Detection of miR-21 and miR-155 Breast Cancer Biomarkers Using a Dual-Signal Aptasensor Based on Cu/Cd Redox Probes
M, with ultralow limits of detection of 62.0 aM for microRNA-21 and 650.0 aM for microRNA-155. The platform demonstrated excellent selectivity, good repeatability and reproducibility, and stable performance for up to 3 weeks. Successful validation in human plasma samples with recovery values between 92% and 119% confirmed the reliability of the proposed system. Overall, this dual-channel biosensor provides a robust and ultrasensitive platform for multiplexed microRNA detection and shows strong potential for early stage breast cancer diagnostics.
Authors
- Ali Ghaffarinejad (ORCID: https://orcid.org/0000-0002-5537-8516)
- Mohammad Mahdi Alemnezhad (ORCID: https://orcid.org/0000-0002-8584-7694)
- Hamid Shahbazmohammadi
Institutions
- Bioanalytica (Switzerland) (CH)
- Center for Non-Communicable Diseases (PK)
- Genetic Analysis (Norway) (NO)
- Qazvin University of Medical Sciences (IR)
- Iran University of Science and Technology (IR)
Publication Details
- Journal
- Langmuir
- Published
- 2026-04-27
- DOI
- https://doi.org/10.1021/acs.langmuir.6c00713
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00