The Specific Low-Interference dsDNA Copper Nanoclusters for Visual Fluorescent Detection and Quantification of the <i>EGFR</i> L858R Point Mutation in Whole Single-Tube Magnetic Purification System
L858R mutation in patients with nonsmall cell lung cancer, yielding an excellent linear calibration curve (r = 0.9981), recovery rates of 95-110%, and a detection limit of 2.33%. These results demonstrate sensitivity comparable to that of commercial qPCR and next-generation sequencing, while offering advantages in instrument cost, simplicity, and analytical specificity.
Authors
- Ravery Sebuyoya (ORCID: https://orcid.org/0000-0002-3769-6676)
- Hwang‐Shang Kou (ORCID: https://orcid.org/0000-0001-7428-0867)
- Chun‐Chi Wang (ORCID: https://orcid.org/0000-0002-3546-9576)
- Ke-Peng Lai
- Kung-Hung Lin (ORCID: https://orcid.org/0000-0003-4290-9282)
Institutions
- Kaohsiung Medical University (TW)
- Kaohsiung Medical University Chung-Ho Memorial Hospital (TW)
- Zuoying Armed Forces General Hospital (TW)
- International Drug Development (FR)
Publication Details
- Journal
- Analytical Chemistry
- Published
- 2026-04-27
- DOI
- https://doi.org/10.1021/acs.analchem.6c00089
- Primary Topic
- Nanocluster Synthesis and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00