Probing Supramolecular Motion with Nuclear Magnetic Resonance in Bioactive Scaffolds that Promote Neural Regeneration in the Central Nervous System
H relaxation data, which suggest pronounced β-sheet structuring within the IKVAV domain. Furthermore, dipolar coupling measurements suggest the possible formation of salt bridges between the E and K residues, folding of PA molecules, and the potential coexistence of parallel and antiparallel molecular arrangements within the dynamic assemblies. X-ray scattering and electron microscopy also revealed a significant population of small PA clusters in equilibrium with micron-length nanofilaments in the more dynamic, bioactive systems, consistent with our previous observations on a related supramolecular system. Based on DOSY NMR, these clusters have a hydrodynamic diameter of 6.6 nm, closely matching the 6.2 nm effective diameter from solution X-ray scattering. Finally, multiphase NMR unambiguously demonstrated the coassembly of PAs bearing the IKVAV moiety with those displaying a peptide that mimics fibroblast growth factor 2, which was necessary to reverse paralysis.
Authors
- Suvendu Biswas (ORCID: https://orcid.org/0000-0002-1348-2358)
- Simon A. Egner (ORCID: https://orcid.org/0000-0001-9430-7844)
- Liam C. Palmer (ORCID: https://orcid.org/0000-0003-0804-1168)
- Radoslav Z. Pavlović (ORCID: https://orcid.org/0000-0003-1216-3950)
- Chunhua Yuan (ORCID: https://orcid.org/0000-0003-2209-4653)
- Mark R. Karver (ORCID: https://orcid.org/0000-0003-2425-7946)
- Tyler J. Jaynes (ORCID: https://orcid.org/0000-0001-6964-0047)
- Hiroaki Sai (ORCID: https://orcid.org/0000-0002-4268-2148)
- Alexandar L. Hansen (ORCID: https://orcid.org/0000-0003-4474-7141)
- Anna Metlushko
- Samuel Isaac Stupp (ORCID: https://orcid.org/0000-0002-5491-7442)
- Songlin Wang (ORCID: https://orcid.org/0000-0002-7588-7377)
- Yaroslav Vorobyov
- Daniel Conroy
- Ethan Singer
Institutions
- Regenerative NanoMedicine (FR)
- High Magnetic Field Laboratory (CN)
- Resonance Research (United States) (US)
- Shanghai Optical Instrument Research Institute (CN)
- The Ohio State University (US)
Publication Details
- Journal
- Journal of the American Chemical Society
- Published
- 2026-04-27
- DOI
- https://doi.org/10.1021/jacs.6c01684
- Primary Topic
- Neuroscience and Neuropharmacology Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Northwestern University
- National Institutes of Health
- Basic Energy Sciences
- National Science Foundation Graduate Research Fellowship Program