Parallelized droplet microfluidic mechanoporation enables robust and clogging-resistant intracellular gene delivery
cells/h, enables highly efficient mRNA transfection (~98%), and supports CRISPR/Cas9-mediated CD3 knock-out. Collectively, these results establish parallelized droplet cell mechanoporation as a scalable and reliable strategy for intracellular delivery with applicability in cell engineering and therapeutic development.
Authors
- Aram J. Chung (ORCID: https://orcid.org/0000-0003-4984-0222)
- Qingluan Liu
Institutions
- Korea University (KR)
- STR Biotech (South Korea) (KR)
- Department of Public (IN)
- Bioengineering Center (RU)
- Korea University (JP)
Publication Details
- Journal
- Microsystems & Nanoengineering
- Published
- 2026-04-28
- DOI
- https://doi.org/10.1038/s41378-026-01273-6
- Primary Topic
- Innovative Microfluidic and Catalytic Techniques Innovation
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Research Foundation
- Korea University
- National Research Foundation of Korea
- Ministry of Science and ICT, South Korea