De novo design of a macrocycle induced dimerization system for cellular control
of 36 nM, and the x-ray crystal structure of the protein homodimer-macrocycle complex is very close to the computational design model, with the C2 axis of the macrocycle aligned with the homodimer C2 axis. Transcriptional and split luciferase assays in mammalian cells demonstrates conditional control over both a reporter gene expression and luciferase reconstitution.
Authors
- Suna Cheng
- Brian Coventry (ORCID: https://orcid.org/0000-0002-6910-6255)
- Asim K. Bera (ORCID: https://orcid.org/0000-0001-9473-2912)
- Patrick J. Salveson (ORCID: https://orcid.org/0000-0003-0962-6010)
- Derrick R. Hicks (ORCID: https://orcid.org/0000-0003-2950-1848)
- Alex Kang (ORCID: https://orcid.org/0000-0001-5487-0499)
- Xinting Li (ORCID: https://orcid.org/0000-0001-5559-7578)
- Carolina Möller
- Stephanie Hanna
- Madison Kennedy
- Meerit Said
- Basile Wicky
- Barry L Stoddard
- Mohamad Abedi
- David Baker
Institutions
- Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa (ZA)
- University of Washington (US)
- Fred Hutch Cancer Center (US)
Publication Details
- Journal
- bioRxiv (Cold Spring Harbor Laboratory)
- Published
- 2026-04-26
- DOI
- https://doi.org/10.64898/2026.04.24.720480
- Primary Topic
- Chemical Synthesis and Analysis
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Howard Hughes Medical Institute