Enhancement of a nuclear factor of activated T cells (NFAT) reporter for the study of G protein-coupled receptors
T cells via CRISPR/Cas9 knock-in. Our NFAT-reporter T cells demonstrate the reporters potential for use in bi-allelic expression systems and primary cell types.
Authors
- Aman Khan (ORCID: https://orcid.org/0000-0003-2760-1490)
- Edward Wills
- Claudia Sisk (ORCID: https://orcid.org/0009-0005-4294-0417)
- Ben Taylor (ORCID: https://orcid.org/0000-0001-6101-3786)
- Graham Ladds (ORCID: https://orcid.org/0000-0001-7320-9612)
- Mona Shehata (ORCID: https://orcid.org/0000-0002-8413-4334)
- Jonathan Sumner
- Anjana Saji
Institutions
- AstraZeneca (United Kingdom) (GB)
- University of Cambridge (GB)
- AstraZeneca (South Korea) (KR)
- AstraZeneca (Singapore) (SG)
Publication Details
- Journal
- Communications Biology
- Published
- 2026-04-25
- DOI
- https://doi.org/10.1038/s42003-026-10110-5
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Takeda Pharmaceuticals U.S.A.
- Royal Society
- Engineering and Physical Sciences Research Council
- Biotechnology and Biological Sciences Research Council