Unusual Acyltransferase Negatively Regulates Nigericin Production via Malonylation of P450 NigD <sub>Sy</sub>
achieved a final titer of 444.5 mg/L, representing a 61.9-fold improvement over the wild-type strain. Our findings reveal a novel lysine malonylation-mediated regulatory mechanism in nigericin biosynthesis and highlight the potential of targeting post-translational modification enzymes for metabolic engineering.
Authors
- Zirong Deng (ORCID: https://orcid.org/0000-0002-8737-9337)
- Chunni Liu
- Zengzhi Liu
- Hui Li (ORCID: https://orcid.org/0000-0001-9198-3951)
- Yunchen Yan
- Weiwei Li (ORCID: https://orcid.org/0000-0002-7329-4236)
- Kaishuai Xing
- Jing Liu
- Fei Xiao
- Xiaoyu Yang
Institutions
- North West Agriculture and Forestry University (CN)
- Qingdao National Laboratory for Marine Science and Technology (CN)
- Ocean University of China (CN)
Publication Details
- Journal
- Journal of Agricultural and Food Chemistry
- Published
- 2026-04-25
- DOI
- https://doi.org/10.1021/acs.jafc.5c12869
- Primary Topic
- Microbial Natural Products and Biosynthesis
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Shandong Province
- Shaanxi Provincial Science and Technology Department