CRISPRi-based functional genomic screening identifies genes essential for CH <sub>4</sub> -dependent growth in a methanotrophic bacterium
. Collectively, our results show that the CRISPRi system and sgRNA library developed here can be used for facile gene-function analyses and genomic screening to identify genetic determinants of methanotrophy and isolation of improved methanotroph biocatalysts.
Authors
- Calvin A. Henard (ORCID: https://orcid.org/0000-0002-4574-587X)
- Rajeev K. Azad (ORCID: https://orcid.org/0000-0002-6874-6146)
- Danyang Shao (ORCID: https://orcid.org/0000-0001-8254-4703)
- Jessica M. Henard
- Yinhui Yu (ORCID: https://orcid.org/0000-0003-1424-7250)
- Spencer A. Lee
Institutions
- University of North Texas (US)
Publication Details
- Journal
- Science Advances
- Published
- 2026-04-24
- DOI
- https://doi.org/10.1126/sciadv.aed4234
- Primary Topic
- Microbial metabolism and enzyme function
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Science Foundation