Post-booster longitudinal plasma proteomic changes following BNT162b2 COVID-19 vaccination in Qatar
Background: The COVID-19 pandemic imposed a major global health and economic burden. Although the pandemic was no longer declared a public health emergency of international concern in May 2023, SARS-CoV-2 variants continue to emerge, and millions remain affected by long COVID. This raises the question whether continued vaccination provides lasting benefits in preventing viral transmission and severe illness. Aim: This longitudinal study assessed the effects of the third BNT162b2 mRNA vaccine dose on the circulating proteome for 6 months. Methods: Plasma levels of 354 unique proteins were quantified before, and at 3- and 6-months post-booster using Olink technology in 70 healthy individuals; 35 infection-naïve and 35 previously infected individuals (18 infected before, 17 after completing the two-dose regimen). Results: , respectively). Conclusions: These findings provide insights into the temporal dynamics of circulating proteomic responses following booster vaccination, highlighting molecular features that may be relevant to immune readiness and post-vaccination inflammatory processes.
Authors
- Abdelilah Arredouani (ORCID: https://orcid.org/0000-0002-5060-916X)
- Adviti Naik (ORCID: https://orcid.org/0000-0001-5147-3869)
- Nehad M. Alajez (ORCID: https://orcid.org/0000-0002-1546-1091)
- Sana Bentebbal (ORCID: https://orcid.org/0000-0001-8686-2434)
- Mohammed Abukhattab (ORCID: https://orcid.org/0000-0002-3335-0563)
- Rowaida Z. Taha (ORCID: https://orcid.org/0000-0001-6119-8389)
- Alberto de la Fuente (ORCID: https://orcid.org/0000-0002-3697-3635)
- Abdullah Aldushain (ORCID: https://orcid.org/0000-0001-7400-4723)
- Hibah Shaath (ORCID: https://orcid.org/0000-0003-3576-7626)
- Ilham Bensmail
- Ali S. Omrani (ORCID: https://orcid.org/0000-0001-5309-6358)
- Neyla S. Al-Akl
- Ahmed Zaqout (ORCID: https://orcid.org/0000-0002-0173-9697)
- Julie Decock (ORCID: https://orcid.org/0000-0002-4409-100X)
- Bakhita Meqbel
- Sara A. Abdulla
- Yongsoo Park
- Muna A. Al-Maslamani
- Abdi Adam
- Omar M. A. El-Agnaf
- Houda Y. A. Moussa
- Remy Thomas
- Houari B. Abdesselem
- Kyung C. Shin
Institutions
- Hamad bin Khalifa University (QA)
- Hamad Medical Corporation (QA)
- Qatar University (QA)
- Qatar Foundation (QA)
Publication Details
- Journal
- Frontiers in Immunology
- Published
- 2026-04-24
- DOI
- https://doi.org/10.3389/fimmu.2026.1762522
- Primary Topic
- SARS-CoV-2 and COVID-19 Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Hamad Medical Corporation
- Qatar Biomedical Research Institute, Hamad Bin Khalifa University