A Simple, Rapid, and Clinically Transferable LC–MS/MS Method for the Detection of Sex Steroid Hormone Metabolic Profiles

> 0.99088. The limits of quantitation of the method were in the range from 0.005 to 1 ng/mL. The recoveries were in the range of 90.4%-107.9% with the intraday and interday precisions within 14.35%. This method had been successfully applied for the determination of SSHs in human serum samples with simple operation and good accuracy and could provide a viable approach for clinical screening of SSHs.