Peptide-enriched hydrogel formulation for sensitive and damaged skin: from design to application testing

Various factors such as infections, wounds, and comorbidities can disrupt the skin's physiological function. Moreover, skin lesions can result from radiation therapy. We aimed to create a new cosmetic formulation with pro-regenerative properties that is specifically designed for patients with sustained skin damage, such as those who have undergone radiation therapy. In the first stage, NE1 and IM2 peptides were synthesized, and hydrogels were prepared. IM2 is a derivative of the IM peptide, while NE1 contains the bioactive GHK sequence. In the next stage physicochemical analyses were conducted, including the evaluation of peptide stability and the developed composition's microbial purity and packaging compatibility. In addition, in vitro safety and activity assessments were implemented on human skin cells. In the final step, dermatological tests were conducted on the participants. The results indicate that the cosmetic composition is stable, possesses preservative properties, and is safe in both in vitro and in vivo studies. Peptide release studies show that within the first hours, approximately 75-80% of each peptide is released, ensuring a rapid onset of action. Analysis of cell migration indicate that both p407 and the designed composition stimulate migration of HaCaT keratinocytes in vitro. Dermatological tests did not show any irritant and sensitizing properties. Application analyses revealed that the designed composition effectively moisturizes and takes care of sensitive skin, alleviates redness and the effects of rough skin, eliminates the sensation of itching and the effects of skin tension, and soothes irritation. This formulation can be used for the daily care of sensitive, allergic, or irritated skin.