Using the Phillygenin Ameliorates the Severe Acute Pancreatitis in Rats by Inhibiting TLR4/NF-κB Pathway

Yiwen Sun,1,2,* Shengze Li,1,2,* Jiming Duan,1,* Jiaxing Li,1,2 Wenxing Li1 1Department of General Surgery, The Second Hospital of Shanxi Medical University, Taiyuan, People’s Republic of China; 2The Second Clinical Medical College of Shanxi Medical University, Taiyuan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Wenxing Li, Department of General Surgery, The Second Hospital of Shanxi Medical University, No. 382, Wuyi St, Taiyuan, 030001, People’s Republic of China, Tel +86 137 0051 5393, Email [email protected]: There is no effective agent against the inflammation in severe acute pancreatitis (SAP). Phillygenin (PHI) exhibits potent anti-inflammatory activity. However, the molecular mechanisms underlying its effects on SAP remain unclear. The aim of the study was to explore the therapeutic effects and regulatory mechanism of PHI on SAP.Methods: Thirty male SD rats were randomly divided into control, SAP and PHI (30 mg/kg) treated groups. The effectiveness of the PHI treatment was determined by looking at the histological scores of the pancreas, the levels of serum amylase and lipase, pathological changes and myeloperoxidase (MPO) activity. The affinity between PHI and the TLR4/NF-κB proteins were predicted using molecular docking. Rat pancreatic AR42J cells were divided into control, cerulein plus LPS and PHI-treated groups (12.5, 25 and 50 μg/mL). In vivo and vitro experiments, the effect of PHI on the release of TNF-α, IL-6, and IL-1β were measured by ELISA. The Western blot and immunohistochemistry were used to uncover the underlying molecular mechanisms targeting on TLR4/NF-κB.Results: PHI treatment significantly improved pancreatic pathology (P < 0.001), decreased the levels of serum amylase and lipase (P < 0.05), reduced pancreatic MPO activity (P < 0.01). Additionally, PHI reduced the release of TNF-α (P < 0.01), IL-6 (P < 0.05), and IL-1β (P < 0.01) in rats. Furthermore, PHI exhibited strong binding ability to both TLR4 and NF-κB. The protein expression of TLR4, NF-κB, and phospho-NF-κB proteins in vivo and in vitro models were downregulated by PHI (P < 0.05). The transfer of phospho-NF-κB to the nucleus was also reduced in the rat pancreas (P < 0.05).Conclusion: PHI exhibits a protective effect against SAP. It can alleviate SAP in rats by inhibiting TLR4/NF-κB pathway. Therefore, PHI may be considered as a novel therapeutic agent against SAP.Keywords: phillygenin, severe acute pancreatitis, inflammation, toll-like receptor 4, nuclear factor-κB