In vivo CRISPR-mediated activation of cardiogenic genes to reprogram cardiac fibroblasts

Background: environment. Methods: adeno-associated viral vectors (AAVs). The reprogramming potential of CFs into cardiovascular lineages was further evaluated in dCas9 mice subjected to myocardial infarction followed by administration of pooled AAVs. Results: -activated CFs exhibited the potential for transdifferentiation into cardiomyocyte-like or vascular smooth muscle cell-like lineages within the infarcted heart, which was not observed in the control group. Conclusion: reprogramming of CFs into cardiovascular cells.