Trypanosomatidae (Kinetoplastea: Trypanosomatida) diversity infecting the South American coati Nasua nasua (Carnivora: Procyonidae) in urban forest fragments in the Brazilian Midwest
Introduction: and included in the epidemiological scenario of Visceral Leishmaniasis in Campo Grande (CG), Brazilian Midwest. Within this context, the aim of this work was to assess the diversity of trypanosomatid species in coatis from CG. Methods: Samples of 110 South American coatis captured in two forest fragments were submitted to Nested Polymerase Chain Reaction (nPCR) of small ribosomal ribonucleic acid subunit (SSU rRNA) gene and Sanger sequencing analyses. Results: and the Molecular Operational Taxonomic Unit Trypanosomatidae sp. CROT. When combining SSU rRNA detection and sequencing with previous published data from the same coati cohort, we observed a positivity rate of 47.3% (52/110), in single (36.4%; n = 40/110) or mixed Trypanosomatidae infections (10.9%; n = 12/110). Discussion: Our findings indicate the South American coati is a key-hosts species in the ecology of trypanosomatids also in the urban environment of CG.
Authors
- Carina Elisei de Oliveira (ORCID: https://orcid.org/0000-0001-9240-4376)
- William Oliveira de Assis (ORCID: https://orcid.org/0000-0003-3327-0800)
- André Luiz Rodrigues Roque (ORCID: https://orcid.org/0000-0002-8740-052X)
- Gabriel Carvalho de Macedo (ORCID: https://orcid.org/0000-0002-4596-5733)
- Wesley Arruda Gimenes Nantes (ORCID: https://orcid.org/0000-0002-3454-4714)
- Andreza Castro Rucco (ORCID: https://orcid.org/0000-0001-5470-3527)
- Geovanna S. Santos
- Leonardo França do Nascimento (ORCID: https://orcid.org/0000-0001-9940-3392)
- Gisele Braziliano de Andrade (ORCID: https://orcid.org/0000-0001-6289-9945)
- Julia Gindri Bragato Pistori (ORCID: https://orcid.org/0000-0001-9352-1048)
- Heitor Miraglia Herrera (ORCID: https://orcid.org/0000-0003-2404-8765)
- Wanessa Gomes Teixeira Barreto
Institutions
- Universidade Federal de Mato Grosso do Sul (BR)
- New York State Department of Environmental Conservation (US)
- Faculdades Oswaldo Cruz (BR)
- Universidade Católica Dom Bosco (BR)
Publication Details
- Journal
- Frontiers in Cellular and Infection Microbiology
- Published
- 2026-04-22
- DOI
- https://doi.org/10.3389/fcimb.2026.1822974
- Primary Topic
- Trypanosoma species research and implications
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro