Rapid and sensitive detection of blaKPC and blaNDM in carbapenemase-producing Enterobacterales using a one-pot recombinase polymerase amplification–CRISPR/Cas12a system with modified protospacer adjacent motifs
within 30 min. Validation with 44 clinical samples demonstrated that the assay had 100% sensitivity and specificity, matching the effectiveness of qPCR. The one-pot RCCS platform offers a robust and highly sensitive POC solution for on-site testing.
Authors
- Jung Sik Yoo
- Sangrim Kang (ORCID: https://orcid.org/0000-0002-4189-427X)
- Songmee Bae (ORCID: https://orcid.org/0000-0002-7423-2360)
- Seung Soon Lee (ORCID: https://orcid.org/0000-0003-1797-3426)
- Lee-Sang Hyeon (ORCID: https://orcid.org/0000-0002-7608-156X)
- Ji Yang (ORCID: https://orcid.org/0009-0001-5320-1098)
- Eunkum Kim
Institutions
- Sacred Heart Hospital (NG)
- LSIS (South Korea) (KR)
- Korea Disease Control and Prevention Agency (KR)
Publication Details
- Journal
- Biosensors and Bioelectronics
- Published
- 2026-04-22
- DOI
- https://doi.org/10.1016/j.bios.2026.118725
- Primary Topic
- Antibiotic Resistance in Bacteria
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Korea National Institute of Health