Bridging the gap: organotypic models to study late-onset group B streptococcus infection
immortalized adult human intestinal cell lines, which can limit the applicability of findings to human neonates. In this study, we introduce the use of three-dimensional fetal tissue-derived human intestinal enteroids and induced pluripotent stem cell-derived human intestinal organoids to study GBS-host interactions within the gut. These novel models demonstrate improved ability to recapitulate the vulnerability of the immature human host and function as a platform to test novel interventional strategies to protect exposed newborns.
Authors
- Hannah L. Wapshott‐Stehli (ORCID: https://orcid.org/0000-0001-7822-9321)
- April K. Lindon
- Rico R. Carter
- Kristen Domínguez (ORCID: https://orcid.org/0000-0001-9085-9406)
- Tara M. Randis (ORCID: https://orcid.org/0000-0003-0348-6766)
- Sophie E. Darch (ORCID: https://orcid.org/0000-0003-2800-2844)
- Thao Ho (ORCID: https://orcid.org/0000-0002-4580-9669)
- Alexia N. Pearah
- N John-Lewis Edwards
- Leigh-Anne M. Worthington
- Madison R. Huszar
Institutions
- University of South Florida (US)
Publication Details
- Journal
- Microbiology Spectrum
- Published
- 2026-04-22
- DOI
- https://doi.org/10.1128/spectrum.02316-25
- Primary Topic
- Neonatal and Maternal Infections
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Institutes of Health