Riboflavin Biosynthesis: Mapping the Tagged Lines of a Complex Metabolic Design

N) that effectively tagged substrate atoms to follow their movement through the pathway. In more recent years, presteady state analyses and high-resolution crystal structures have brought sharper focus to the proposed enzymatic mechanisms, adding depth and shading to the emerging picture. The purpose of this article is to define the current state of our understanding of the enzymatic mechanisms that compose the riboflavin biosynthetic pathway, bringing the latest evidence together as an updated rendering of this intricate biochemical (master)piece.