Restoring redox homeostasis through S-adenosyl-L-methionine and B-vitamins co-supplementation alleviates ethanol induced hepato-pancreatic injury by regulating glutathione biosynthesis in C57BL/6J mice

Background: Alcohol use disorder(AUD) is a chronic condition responsible for more than 3 million deaths annually worldwide and contributes to the development of multiple systemic comorbidities. Chronic alcohol consumption promotes inflammatory responses, immune suppression, antioxidant dysregulation, and nutritional deficiencies, severely affecting organs such as the liver and pancreas. Disruption of methionine metabolism and antioxidant defenses, including reduced availability of S-adenosyl-L-methionine (SAMe) and essential B-vitamins, has been implicated in alcohol-induced oxidative stress. Therefore, the aim of the present study was to investigate whether co-supplementation with SAMe and B-vitamins could alleviate ethanol-induced hepato-pancreatic injury by improving redox homeostasis and glutathione biosynthesis. Methods: ) were analysed to corroborate the findings. Results: < 0.001), collectively indicating a protective therapeutic effect. Conclusion: Chronic ethanol exposure induces profound oxidative stress and impairs endogenous antioxidant defence system, culminating in severe hepato-pancreatic injury. Restoration of glutathione homeostasis and stabilization of cellular redox balance through SAMe and B-vitamins co-supplementation efficiently attenuated organ pathology, thereby improving the overall wellbeing of the affected individual.