Comparative evaluation of the remineralisation potential of biomimetic agents on phosphorylated dentine using SEM–EDX analysis: an in vitro study

Introduction: Dentine remineralisation remains a clinical challenge due to its higher critical pH and complex organic matrix. Biomimetic strategies that replicate the role of non-collagenous proteins through phosphorylation have shown promise in promoting intrafibrillar mineralisation. Sodium tripolyphosphate (STPP) has been proposed as a phosphorylating agent capable of enhancing dentine remineralisation. This in vitro study aimed to comparatively evaluate the remineralisation potential of different biomimetic agents on artificially demineralised dentine using scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX) analysis. Methodology: = 10). Remineralisation protocols were carried out for 7 and 21 days, followed by SEM and EDX evaluation. Results: < 0.05). Conclusion: Phosphorylation of dentine using STPP enhances the remineralisation efficacy of biomimetic agents. CPP-ACP in combination with STPP showed superior remineralisation potential. These findings support the use of phosphorylating biomaterials as promising strategies for dentine remineralisation. However, the findings are limited by the in vitro design and require clinical validation.