Harnessing 3-Fucosyllactose: Dynamic Regulation Unlocks Efficient <scp>l</scp> -fucose Production in <i>Escherichia coli</i>
subsp. We knocked out competing pathways and balanced the expression of modular enzymes. Additionally, a temperature-coupled mixed carbon source strategy and dynamic metabolic control were employed to decouple cell growth from product synthesis, thereby directing metabolic flux toward l-fucose production. In a 5 L fed-batch system, the engineered strain achieved an l-fucose titer of 86.22 g/L within 89 h. This stable, plasmid-free and antibiotic-free system provides a new way for the safe and large-scale production of l-fucose.
Authors
- Jianmin Wu (ORCID: https://orcid.org/0000-0002-0999-9194)
- Jianming Yao (ORCID: https://orcid.org/0009-0002-1007-0689)
- Zhiqiang Huang (ORCID: https://orcid.org/0000-0002-5562-8407)
- Caiwen Lao
- Xiangsong Chen
- Lixia Yuan
Institutions
- University of Science and Technology of China (CN)
- Anhui University (CN)
- Hefei University of Technology (CN)
- Chinese Academy of Sciences (CN)
- Hefei Institutes of Physical Science (CN)
- Czech Academy of Sciences, Institute of Plasma Physics (CZ)
- Centre of Plasma Physics - Institute for Plasma Research (IN)
Publication Details
- Journal
- Journal of Agricultural and Food Chemistry
- Published
- 2026-04-22
- DOI
- https://doi.org/10.1021/acs.jafc.5c17430
- Primary Topic
- Escherichia coli research studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00