Pangenome Architecture and Accessory Gene-Driven Population Structure of Staphylococcus aureus Revealed by a Hospital-Adjacent Environmental Isolate
. The core genome stabilized at approximately 1757 genes, including 1651 genes conserved across all genomes. Gene frequency spectra showed a dominant cloud genome and a structured shell fraction contributing to interstrain differentiation. Jaccard-based gene content similarity resolved clusters shaped mainly by accessory gene composition. The environmental isolate retained the complete core genome, carried only 15 isolate-specific gene families (0.18% of the pangenome), and clustered within an established lineage. Its unique content included a lincosamide resistance-associated locus and efeB, a gene potentially related to heme or iron metabolism and oxidative stress response. These findings highlight a conserved genomic backbone over a dynamic accessory reservoir and support One Health genomic surveillance that includes wildlife-associated niches, while indicating that the environmental isolate fits within the broader gene content diversity observed in the analyzed dataset.
Authors
- Camila Botelho Miguel (ORCID: https://orcid.org/0000-0002-1834-1394)
- Christophe Morisseau (ORCID: https://orcid.org/0000-0002-5672-6631)
- Mariana Santos Cardoso (ORCID: https://orcid.org/0000-0002-4351-5835)
- Wellington Francisco Rodrigues (ORCID: https://orcid.org/0000-0002-3426-2186)
- Renata Botelho Miguel
- C Renato M. Oliveira
- Jamil Miguel Neto (ORCID: https://orcid.org/0000-0002-7024-1294)
- Ferdinando Agostinho
- Melissa Carvalho Martins-de-Abreu
- Sabrina Martins Calegari
- Laise Mazurek
- Geovana Pina Vilela
- Karen M. Wagner
- Siomar Soares
- Carlos Ueira-Vieira
- Aristóteles Góes-Neto
- Amanda Bertinetti Tres
Institutions
- Universidade Federal de Minas Gerais (BR)
- Universidade Federal do Triângulo Mineiro (BR)
- Universidade de Rio Verde (BR)
- UC Davis Comprehensive Cancer Center
- Universidade Federal de Uberlândia (BR)
- University of California, Davis (US)
Publication Details
- Journal
- Microorganisms
- Published
- 2026-04-21
- DOI
- https://doi.org/10.3390/microorganisms14040938
- Primary Topic
- Antimicrobial Resistance in Staphylococcus
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Universidade Federal de Uberlândia
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Fundação de Amparo à Pesquisa do Estado de Minas Gerais
- Universidade Federal de Minas Gerais
- University of California, Davis