An Orc1 initiator-specific motif (ISM)-related region limits ORC–ssDNA binding and promotes replication origin specificity in budding yeast
, indicating a redistribution of ORC from replication origins to ssDNA-forming loci. Despite conservation of the ISM across domains of life, these results suggest that eukaryotic ORC has functionally diverged such that origin binding is coupled to the repression of ssDNA binding through the Orc1 ISM-related region, thereby safeguarding faithful origin selection.
Authors
- Takuya Kurihara (ORCID: https://orcid.org/0000-0002-0882-0906)
- Takeaki Chichibu
- Ryuya Muraoka
- Hironori Kawakami (ORCID: https://orcid.org/0000-0001-8971-1914)
- Tsutomu Katayama (ORCID: https://orcid.org/0000-0001-9994-1684)
- Eiji Ohashi (ORCID: https://orcid.org/0000-0002-1771-5585)
- Shota Kanamoto
- Kanako Asada
Institutions
- Kyushu University (JP)
- Sanyo-Onoda City University (JP)
Publication Details
- Journal
- Frontiers in Microbiology
- Published
- 2026-04-22
- DOI
- https://doi.org/10.3389/fmicb.2026.1778270
- Primary Topic
- DNA Repair Mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 0.00