CRISPRi and beyond: studying essential gene function in the obligate intracellular bacterium <i>Chlamydia trachomatis</i>
. These include systems to conditionally knock down or knockout a target gene product using CRISPR interference (CRISPRi), inducible small RNAs (sRNA), fluorescence-reported allelic exchange mutagenesis (FRAEM) with inducible complementation of the target gene, and dependence on plasmid expression (DOPE).
Authors
- Adarsh Gopinath
- Scot P. Ouellette (ORCID: https://orcid.org/0000-0002-3721-6839)
- Li Shen (ORCID: https://orcid.org/0000-0001-9869-7317)
Institutions
- University of Nebraska Medical Center (US)
- Louisiana State University Health Sciences Center New Orleans (US)
Publication Details
- Journal
- Journal of Bacteriology
- Published
- 2026-04-22
- DOI
- https://doi.org/10.1128/jb.00059-26
- Primary Topic
- Reproductive tract infections research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Institutes of Health