Combinatorial Engineering of the Catalytic Pocket and Structural Stability in 4-Vinylguaiacol Oxygenase for Efficient Vanillin Biosynthesis
catalysis of ferulic acid yielded 19.3 g/L vanillin within 20 h, with a maximum rate of 9.8 mM/h.
Authors
- Yujie Feng (ORCID: https://orcid.org/0000-0002-0686-3937)
- Jian Tian (ORCID: https://orcid.org/0000-0002-9997-6518)
- X X Xu (ORCID: https://orcid.org/0000-0001-6674-1811)
- Lingxi Fan
- Xiaofen Fu
- Qifeng Wen
- Jianmin Xing
- Jinlong Liu
- Qinghong He
Institutions
- National Agricultural Research Institute (PG)
- Chinese Academy of Sciences (CN)
- Hebei University of Science and Technology (CN)
- Jiangsu Food and Pharmaceutical Science College (CN)
- Institute of Process Engineering (CN)
- Chinese Academy of Agricultural Sciences (CN)
- Biotechnology Research Institute (CA)
- Institute of Animal Sciences (CN)
- Kai Biotech (South Korea) (KR)
- Innovative Research (United States) (US)
- Institute for Fermentation (JP)
- University of Chinese Academy of Sciences (CN)
- State Key Laboratory of Animal Nutrition
Publication Details
- Journal
- Journal of Agricultural and Food Chemistry
- Published
- 2026-04-22
- DOI
- https://doi.org/10.1021/acs.jafc.6c00484
- Primary Topic
- Biochemical and biochemical processes
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China