BPP Bioportide™-mediated (genetic) transformation in cyanobacteria: a rapid and simplified approach for efficient molecular translocation and genome modification
sp. UTEX 3153. BPP Bioportide™ variants BP-17 and BP-12 significantly improved transformation efficiency of both plasmid and linear DNA, even with minimal DNA input of 10 ng, surpassing conventional methods and enabling modification of previously non-model strains. Optimization revealed a narrow window for DNA uptake and identified key factors influencing genomic integration, such as DNA type, host ploidy, and selection conditions. Successful double homologous recombination and partial to full genomic segregation were validated by colony PCR. BPP Bioportide™-mediated transformation offers a versatile and efficient platform for cyanobacterial genome engineering, supporting advances in synthetic biology and carbon-neutral biotechnology.
Authors
- Karl Forchhammer (ORCID: https://orcid.org/0000-0003-3199-8101)
- Soumila Mondal (ORCID: https://orcid.org/0000-0002-4627-3544)
- Sofia Doello
- Janette T. Alford
- Christoph Kutzner
Institutions
- Washington University in St. Louis (US)
- Université Paris-Saclay (FR)
- Peptide Protein Research (United Kingdom) (GB)
- Institut de Biologie Intégrative de la Cellule (FR)
- Human Computer Interaction (Switzerland) (CH)
Publication Details
- Journal
- Frontiers in Plant Science
- Published
- 2026-04-22
- DOI
- https://doi.org/10.3389/fpls.2026.1812316
- Primary Topic
- Photosynthetic Processes and Mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 0.00