A Novel Microfluidic System for Immunomagnetic Isolation of Pancreatic Cancer CTCs: Plectin-1 and EpCAM as Potential Tumor Markers
cells/ml (47 ± 9% recovery), and EpCAM at 1.25 μg/mL achieved a 92 ± 8% recovery. The entire isolation process from biological samples was performed in less than 1 min. Using this system combined with antibody-functionalized magnetic nanoparticles targeting EpCAM and Plectin-1, we demonstrated the efficient capture of PDAC cells from biological samples, highlighting Plectin-1 as a promising PDAC marker and the potential of immunomagnetic microfluidic separation for improved diagnostics.
Authors
- Michał Skibniewski (ORCID: https://orcid.org/0000-0002-9578-2476)
- Elżbieta Jastrzębska (ORCID: https://orcid.org/0000-0002-9371-9166)
- Jakub Trzciński (ORCID: https://orcid.org/0000-0001-5556-2690)
- Dominik Kołodziejek (ORCID: https://orcid.org/0000-0003-0335-2661)
- Patrycja Baranowska (ORCID: https://orcid.org/0000-0003-1010-2441)
- Weronika Świtlik
- Bartłomiej Łuszczuk
Institutions
- Warsaw University of Technology (PL)
- Warsaw University of Life Sciences (PL)
- University of Warsaw (PL)
Publication Details
- Journal
- ACS Omega
- Published
- 2026-04-22
- DOI
- https://doi.org/10.1021/acsomega.5c12181
- Primary Topic
- Skin and Cellular Biology Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Ministerstwo Edukacji i Nauki