TissuStamp: a novel high-throughput tissue transfer workflow for FFPE spatial biology assays

of tissue area) onto a single standard 75 mm × 25 mm slide in a tightly packed, multi-lane array format. TissuStamp allows tissue cutting, storage and transfer to occur at different times and locations, facilitating multi-institutional studies and biobank initiatives. While TissuStamp was developed for the G4X™ platform, which performs integrated spatial transcriptomic, proteomic, and fluorescent H&E (fH&E™) profiling on up to 128 samples per run at subcellular resolution, its capabilities are transferable to other tissue analysis workflows. Comparative analysis demonstrated that TissuStamp preserved tissue morphology and delivered equivalent multiomic assay performance compared to conventional direct mounting onto slides. This method provides a practical solution for scaling spatial multiomics to cohort-scale research and clinical applications.