Stabilization of transaminases in aqueous-organic media by pyridoxal-5’-phosphate: a case study of transaminase from <i>Desulfomonile tiedjei</i>

), both in incubation mode and under reaction conditions. Particular attention is paid to the analysis of the effect of the amino acid substitution T199Q in the cofactor-binding region on the enzyme's resistance to organic solvents. The present study contributes to addressing the practical problem of stabilizing transaminases in aqueous-organic media. The results also deepen our understanding of the molecular basis of the stability of PLP-dependent enzymes.