Online Deglycosylation of Monomeric Intact Proteins Using the PNGase Rc Immobilized-Enzyme Reactor
-hHD6, bevacizumab, and trastuzumab as model substrates. The results demonstrate that the PNGase Rc IMER enables rapid and efficient online deglycosylation of glycoproteins and antibodies bearing complex sialylated and core-fucosylated glycans, highlighting its potential in bioanalytical workflows, quality control during biopharmaceutical development, and stability tests.
Authors
- Katarína Molnárová
- Petr Novák (ORCID: https://orcid.org/0000-0001-8688-529X)
- Petr Pompach (ORCID: https://orcid.org/0000-0002-0606-0851)
- Jana Nováková (ORCID: https://orcid.org/0000-0001-6771-4225)
Institutions
- Czech Academy of Sciences (CZ)
- Contipro (Czechia) (CZ)
- Czech Academy of Sciences, Institute of Microbiology (CZ)
Publication Details
- Journal
- ACS Omega
- Published
- 2026-04-21
- DOI
- https://doi.org/10.1021/acsomega.6c00577
- Primary Topic
- Glycosylation and Glycoproteins Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- European Commission
- Ministerstvo Školství, Mládeže a Tělovýchovy