Construction and characterization of novel <i>Mycobacterium tuberculosis</i> -derived triple and quadruple knockout vaccines against tuberculosis
), were evaluated for their immunogenicity and safety in mice. Whereas TKO-Z and QKO strains exhibited superior immunogenicity compared to the DKO strain, their protective efficacy in mice was comparable. However, survival studies involving SCID mice indicated that the QKO strain was highly attenuated. Therefore, rational deletion of genes in Mtb seems to be an innovative approach for developing safer and more efficacious vaccines against TB.
Authors
- Vivek Chauhan (ORCID: https://orcid.org/0000-0002-4189-8481)
- Abhishek Mishra (ORCID: https://orcid.org/0000-0003-2077-0937)
- Subramanian Dhandayuthapani (ORCID: https://orcid.org/0009-0001-4390-3811)
- Kishore Das
- Chinnaswamy Jagannath (ORCID: https://orcid.org/0000-0002-3014-2857)
- Areanna Carmona
- Shrikanth S. Gadad (ORCID: https://orcid.org/0000-0003-0030-5472)
- Jessica Chacon
- Varsha Rawat
- Omar Garnica
- Arshad Khan
- Rakesh Kumar
- Enrique I. Ramos
- Raja Veerapanidan
Institutions
- Texas Tech University (US)
- The University of Texas at El Paso (US)
- Cornell University (US)
- University Medical Center of El Paso (US)
- Methodist Hospital (US)
- Methodist Hospital (US)
- Texas Tech University Health Sciences Center (US)
Publication Details
- Journal
- Infection and Immunity
- Published
- 2026-04-21
- DOI
- https://doi.org/10.1128/iai.00500-25
- Primary Topic
- Tuberculosis Research and Epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- American Cancer Society
- National Institute of General Medical Sciences
- National Institute of Allergy and Infectious Diseases